Trump cited concerns over illegal immigration and Fentanyl trafficking as the reason for the tariffs.
Bruce Burrows is the CEO of the Chamber of Marine Commerce, a group representing Canadian and American shipping stakeholders.
He says the new tariffs might hurt Agriculture companies he represents on both sides of the border.
"They manufacture products that they sell to the American consumer. And if their input costs go up by 25 percent then that's gonna be passed on very quickly to the US consumer. And so this is, this is not good."
Trump and Canadian Prime Minister Justin Trudeau have discussed the tariffs.
Last year, the Great Lakes-St Lawrence Seaway generated over 50 billion U.S Dollars in economic activity